2013 - CTS 2013 Congress

This page contains exclusive content for the member of the following sections: TTS, CTS. Log in to view.

Oral Communications 5

12.3 - An isolated venous sac as a suitable place for pancreatic islet transplantation

Presenter: Ekaterine, Berishvili, Tbilisi, Georgia
Authors: Ekaterine Berishvili1,2,3,4,5,6, Zurab Kakabadze1,2,3,4,5,6, Camillo Ricordi1,2,3,4,5,6, AM James Shapiro1,2,3,4,5,6, Sanjeev Gupta1,2,3,4,5,6, Koba Shanava1,2,3,4,5,6

An isolated venous sac as a suitable place for pancreatic islet transplantation.

Ekaterine Berishvili1,2,3,4,5,6, Zurab Kakabadze1,2,3,4,5,6, Camillo Ricordi1,2,3,4,5,6, AM James Shapiro1,2,3,4,5,6, Sanjeev Gupta1,2,3,4,5,6, Koba Shanava1,2,3,4,5,6

1Department of Clinical Anatomy, Tbilisi State Medical University, Tbilisi, Georgia; 2Division of Cell transplantation, Georgian National Institute of Medical Research, DRI Federation, Tbilisi, Georgia; 3Tissue Bioengineering and Stem Cell Center , Ilia State University, Tbilisi, Georgia; 4Cell Transplant Center, Diabetes Research Institute, University of Miami, DRI Federation , Miami, FL, United States; 5Clinical Islet Transplant Program and Department of Surgery, , University of Alberta, DRI Federation , Edmonton, AB, Canada; 6Departments of Medicine and Pathology, Diabetes Center, Marion Bessin Liver Research Center, Cancer Center, Gottesman Institute for Stem Cell Research and Regenerative Medicine, Albert Einstein College of Medicine, Bronx, NY, United States

 

Background: Cell therapy with transplantation of pancreatic islets is of significant interest for type-1 diabetes mellitus. Although transplanted islets survive after intraportal injection, limited engraftment efficiency and eventual loss of transplanted islets constitutes a major limitation, and alternative approaches are required. We evaluated the feasibility of minimal islet mass transplantation into the isolated venous sac with the potential to correct hyperglycemia in diabetic rats.
Methods: Pancreatic islets were isolated from adult Lewis rats and transplanted into either isolated venous sac or intraportaly in diabetic syngeneic rats. The engraftment, survival and function of transplanted islets was determined by histological analysis of tissues, as well as by glycemic control in animals. Results: Islets transplanted in isolated venous sac restored euglycemia in diabetic rats, in contrast animals with intraportal transplantation of islets remained diabetic. Morphological studies revealed
that islets transplanted in isolated venous sac had normal morphology, and maintained expression of insulin. Removal of islet graft-bearing venous segments in diabetic rats led to recurrence of hyperglycemia. Conclusions: We demonstrated that minimal mass of pancreatic islets can be successfully engrafted into the isolated venous sac and restore euglicemia in STZ diabetic rats. Transplantation of pancreatic islets into the isolated venous sac would prove a major step forward, can be clinically applicable and eventually can solve problems encountered with the intrahepatic islet transplantation.

Important Disclaimer

By viewing the material on this site you understand and accept that:

  1. The opinions and statements expressed on this site reflect the views of the author or authors and do not necessarily reflect those of The Transplantation Society and/or its Sections.
  2. The hosting of material on The Transplantation Society site does not signify endorsement of this material by The Transplantation Society and/or its Sections.
  3. The material is solely for educational purposes for qualified health care professionals.
  4. The Transplantation Society and/or its Sections are not liable for any decision made or action taken based on the information contained in the material on this site.
  5. The information cannot be used as a substitute for professional care.
  6. The information does not represent a standard of care.
  7. No physician-patient relationship is being established.

Social

Contact

Staff Directory
+1-514-874-1717
info@tts.org

Address

The Transplantation Society
International Headquarters
740 Notre-Dame Ouest
Suite 1245
Montréal, QC, H3C 3X6
Canada

Info

Privacy Policy
Terms of Use
Copyright Notice