2010 - Transplantomics and Biomarkers in Transplantation


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MINI-ORAL PRESENTATIONS

4.10 - AUTOANTIGEN BIOMARKER DISCOVERY THROUGH IMMUNOLOGICAL...

Presenter: Thien, Dinh, , USA
Authors: Dawn Mattoon, Mary Brodey, Gengxin Chen, Barry Schweitzer, Thien Dinh, Dhavel Patel.

AUTOANTIGEN BIOMARKER DISCOVERY THROUGH IMMUNOLOGICAL PROFILING WITH FUNCTIONAL PROTEIN MICROARRAYS
Dawn Mattoon1, Mary Brodey1, Gengxin Chen1, Barry Schweitzer1, Thien Dinh1, Dhavel Patel2. 1Life Technologies, Cupertino, CA; 2Novartis; USA.
The diagnostic value of serum autoantibodies for many diseases, including cancer, diabetes, and autoimmune disorders is well established. Identifying the antigens that elicit an autoimmune response can yield panels of biomarkers that can be used as classifiers for particular diseases, disease stages, or as predictors of patient outcomes. The present study utilized high content protein microarrays comprised of more than 5,000 purified full-length human proteins, including a panel of 25 known autoantigens, to evaluate immunological profiles across panels of serum samples derived from healthy donors and Systemic Lupus Erythemasosus (SLE) patients. Three statistical algorithms were applied to analyze data from individual microarrays, to compare data between populations and identify candidate biomarkers. This line of investigation identified a panel of 18 novel biomarkers which could differentiate SLE patients from healthy individuals more accurately than a panel of 10 established SLE biomarkers. Studies utilizing the Luminex platform and a custom-printed protein microarray were used to validate the results obtained from the ProtoArray . Taken together, our results suggest that functional protein microarray technology is a powerful new tool for the rapid discovery of autoantigen biomarkers.


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