Objective: We have proved that omentum was a more efficient site to perform non-purified islet transplantation with more safety in Beagle dog islet auto-transplantation model. In this study, we have investigated the outcome of intraomental non-purified islet transplantation.
Methods: 2 patients with type 1 diabetes were transplanted with non-purified islet isolated from DCD donor pancreas by laparoscopic approach and resorbable plasma-thrombin was used as the biologic scaffold in this procedure.
Results: These two patients required about 50 IU exogenous insulin with fasting C-peptide <0.01 ng/mL pre-transplantation. Non-purified islet isolate from DCD donor pancreases as describe in table 1, both patient's insulin requirement was decreased to 20-30 IU/day, and the level of fasting C-peptide was increase to 0.37 and 0.62 ng/ml respectively 3 weeks post transplantation, HbA1c level was decteased from 6.9% to 5.7% and 8.1% to 7.1% respectively 2 month after islet transplantation. There were no complications occured in these two recipients after the islet transplant procedure.
Conclusion: The procedure of laparoscopic intraomental islet transplantation is a safe and effective technique route for clinical islet transplantation, using non-purified islet in this procedure could improve the successful rate of DCD donor islet isolation for clinical transplantation.