2011 - IPITA - Prague
This page contains exclusive content for the member of the following sections: TTS, IPITA. Log in to view.
Poster
1.262 - Cell aggregate formation of islets and sertoli cells
Presenter: N. , Takemoto, ,
Authors: H. Iwata, N. Takemoto, Y. Teramura
P-262
Cell aggregate formation of islets and sertoli cells
H. Iwata, N. Takemoto, Y. Teramura
Kyoto University, Institute for Frontier Medical Sciences, Kyoto, Japan
Sertoli cells which play a crucial role in creating the immunoprivileged environment of the testis has been examined to realize a long term survival of islets of Langerhans by their cotransplantation. Sertoli cells should exist near islets to protect the graft from rejection. In this study, conjugates of single stranded oligonucleotide (ssDNA), poly(ethylene glycol) and phospholipid (ssDNA-PEG-lipid) were used to immobilize Sertoli cells on islets. Oligo-adenine and oligo-thymine were used as ssDNAs and were presented on surfaces of Sertoli cells and islets, respectively, through the hydrophobic interaction between a lipid unit of the conjugates and the cell membrane. The Sertoli cells could be immobilized on the islets through hybridization between oligo-adenine and oligo-thymine. When Sertoli cell-immobilized islets were infused through the portal vein into liver of mice, Sertoli cells remained around islets.
Parallel poster session 7 - Topic: Pancreas transplantation 2
/P-262
Cell aggregate formation of islets and sertoli cells
H. Iwata, N. Takemoto, Y. Teramura
Kyoto University, Institute for Frontier Medical Sciences, Kyoto, Japan
Sertoli cells which play a crucial role in creating the immunoprivileged environment of the testis has been examined to realize a long term survival of islets of Langerhans by their cotransplantation. Sertoli cells should exist near islets to protect the graft from rejection. In this study, conjugates of single stranded oligonucleotide (ssDNA), poly(ethylene glycol) and phospholipid (ssDNA-PEG-lipid) were used to immobilize Sertoli cells on islets. Oligo-adenine and oligo-thymine were used as ssDNAs and were presented on surfaces of Sertoli cells and islets, respectively, through the hydrophobic interaction between a lipid unit of the conjugates and the cell membrane. The Sertoli cells could be immobilized on the islets through hybridization between oligo-adenine and oligo-thymine. When Sertoli cell-immobilized islets were infused through the portal vein into liver of mice, Sertoli cells remained around islets.
Parallel poster session 7 - Topic: Pancreas transplantation 2
Important Disclaimer
By viewing the material on this site you understand and accept that:
- The opinions and statements expressed on this site reflect the views of the author or authors and do not necessarily reflect those of The Transplantation Society and/or its Sections.
- The hosting of material on The Transplantation Society site does not signify endorsement of this material by The Transplantation Society and/or its Sections.
- The material is solely for educational purposes for qualified health care professionals.
- The Transplantation Society and/or its Sections are not liable for any decision made or action taken based on the information contained in the material on this site.
- The information cannot be used as a substitute for professional care.
- The information does not represent a standard of care.
- No physician-patient relationship is being established.
Contact
Address
The Transplantation Society
International Headquarters
740 Notre-Dame Ouest
Suite 1245
Montréal, QC, H3C 3X6
Canada